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Induction of endogenous expression of ZKSCAN3 with CRISPR-dCas9 transcriptional activation system
Induction of endogenous expression of ZKSCAN3 with CRISPR-dCas9 transcriptional activation system ZKSCAN3 +/+ and ZKSCAN3 -/ – hMSCs were collected enzymatically by TrypLE (Gibco) and centrifuged at 1500 grams for 5 min at RT. The pellets were repaired with 4per cent glutaraldehyde in PBS at 4A°C instantly. Products were dehydrated in a graded series of… Continue reading Induction of endogenous expression of ZKSCAN3 with CRISPR-dCas9 transcriptional activation system